A direct competitive ELISA was designed to detect Chloramphenicol residues in samples. The detection limit for Chloramphenicol is 0.05 ppb and there is no cross reaction to other antibiotics and sulfa drugs. The whole procedures can be carried out in 90 minutes.
Specificity	No cross reactivity with other antibiotics.
Sensitivity of Samples	Matrix	Sensitivity (ppb)
	Milk	0.1
	Honey, Royal Jelly	0.1
	Fish, Shrimp, Crab, Squid, Chicken meat and pork	0.025
	Serum	0.025
	Mixed Feed	0.25
	Egg	0.025
Recovery rate	Sample	Recovery rate
	Milk (0.25 ppb)	70~110%
	Honey (0.1 ppb)	90~130%
	Honey (0.3 ppb)	90~130%
	Royal Jelly (0.3 ppb)	80~120%
	Fish, Shrimp, Crab, Squid, Chicken meat and pork(0.1 ppb)	75~125%
	Fish, Shrimp, Crab, Squid, Chicken meat and pork(0.3 ppb)	75~125%
	Serum (0.3 ppb)	80~120%
	Mixed Feed (2.0 ppb)	90~130%
	Egg (0.3 ppb)	80~120%
Operation Benefit	Operation time : 90 mins

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